A New solid-state NMR Homonuclear Correlation Method and its Application for Detecting Nucleotides and Their Hydrogen Bonds in Intact Viral RNA

Orr Simon Lusky – @ItIsLusk

My contribution to the Global NMR Twitter Conference. Please share your thoughts about the work I've done under the supervision of professor Amir Goldbourt, school of chemistry, Tel Aviv University #GlobalnmrTC2022 #solid_state_NMR #RNA @GoldbourtNMRlab @chemistrytau pic.twitter.com/crOAoAkybR

— Orr Simon Lusky (@ItIsLusk) August 3, 2022

We present a new solid state NMR method we developed for studying biological macromolecules called ‘Pulse induced resonance with angular dependent total enhancement’ (PIRATE). We demonstrate a new resonance condition, and the application on finding hydrogen bond patterns in intact viral RNA harvested directly from the MS2 bacteriophage.