Bijaylaxmi Patra – @BijaylaxmiNMR
Glycosaminoglycans (GAGs) and protein interactions are essential in numerous biochemical processes involving ion-pair interactions. However, there is no evidence of this, in native cartilage. Using dynamic nuclear polarization (DNP)-enhanced magic-angle spinning (MAS) NMR, we detected these interactions, revealing charge-pair salt-bridges and potential hydrogen bonding, offering new structural insights.
Mayra Paola Oquist Phillips – @oquist_paola
Glycoprotein 120 (gp120) shields the HIV virus using high mannose glycans, which are difficult to analyze structurally due to their high isochronicity causing NMR signal collapse. Novel NMR methods, including paramagnetic probes, now enable detailed study of gp120 and its interaction with the neutralizing antibody 2G12.
Orr Lusky – @ItIsLusk
We present a robust method using ssNMR to characterise the protein dynamics in an intact virus using pseudo 3D experiments. We show an automated protocol to quantify site-specific order parameters in the backbone, and demonstrate it on the fd-Y21M bacteriophage.
Alexandra Gloria – @_AGloria
Feedlot cattle are crucial to Australia’s livestock industry, supplying beef for domestic and export markets. High summer temperatures and humidity increase heat stress vulnerability, impacting growth, productivity, and welfare. We use NMR-based metabolomics and clinical biochemistry to understand the metabolic effects of heat stress to help mitigate its impact.
Somnath Mondal – @somomsom
The picornaviral 3C(D) protein is a vital protease that cleaves viral polyproteins and host cell defense proteins, interacting with replication elements to control replication and translation. Its interaction with RNA, causing liquid-liquid phase separation, could influence viral apoptosis and infection in the host cells.
Julia Pakuła – @x_yuliya_x
Emerging as a promising area in anticancer research, noncanonical DNA structures such as G-quadruplexes are studied using NMR spectroscopy. NOESY spectra reveals crucial interactions between ligands and G-quadruplexes, helping to understand non-covalent binding mechanisms. This knowledge is essential for rational drug design targeting these structures.
Sarah Walsh – @SarahW29329
Wolbachia, a common endosymbiont of insects, exerts significant antiviral effects. This phenomenon is valuable in reducing the spread of arboviruses, such as dengue. Employing NMR metabolomics, we uncover that in virus-infected Drosophila melanogaster Wolbachia drives host metabolism in a way that inhibits RNA virus replication both directly and indirectly.
Valentina Sobornova – @VSobornova7121
We analyzed the conformational equilibria of flufenamic acid in various media using 2D NOESY spectroscopy and quantum chemical calculations. Our results demonstrate that supercritical carbon dioxide (scCO2) is an effective medium for altering and controlling the conformational preferences of slightly soluble compounds.
Konstantin Belov – @Konstantin94484
In our study, we investigated the conformer distributions of lidocaine under various isobaric and isothermal conditions. Utilizing quantum chemical calculations and NOESY spectroscopy, we found that trans-conformers, stabilized by intramolecular hydrogen bonds, predominate under specific conditions. These findings significantly influence the formation of lidocaine dimers.
Kelsey Marr – @kelsey_marr
Deep eutectic solvents (DES) are low-cost, no-waste mixtures made of hydrogen bond acceptors (HBA) and hydrogen bond donors (HBD). One of the most common DES is ethaline, 1:2 choline chloride to ethylene glycol. The aim of this work is to fully elucidate the hydrogen bond network structure by 2D NMR, specifically through Nuclear Overhauser spectroscopy (NOESY). Previous attempts for ethaline have been hindered by multiple effects, one of the most prominent being radiation dampening. We have successfully utilized a NO Radiation Dampening (NORD) pulse sequence modification to the NOESY sequence and obtained build up curves of NOE and of chemical exchange.
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