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Understanding antimicrobial activity in live cells
Ángela Serrano Sánchez – @Angelasrno99
We have optimised a new, simple methodology to assess membrane permeability of drugs making use of the paramagnetic relaxation enhancement (PRE) effect in lipid vesicles that is applicable to any type of cell membrane. Using this assay, we have been able to find important differences in the permeability of new families of antimicrobial compounds, providing unique information for the further development of this family of compounds. In addition, we have now expanded this assay to live cells making use of a NMR-coupled bioreactor. Using this set up, we are to monitor both the drug permeation into live cells, as well as changes in the bacterial metabolism as a result of the antimicrobial agent added.
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Ligand Field, Concentration and Temperature Paramagnetic Effects on Diruthenium Cores Using p-NMR
Almudena Inchausti – @AlmuInchausti
The [Ru2Cl(Dp-FPhF)x(O2CCH3)4-x] series has been synthesized to study the gradual effects of ligand substitution. Regarding this, p-NMR measurements helped us determine paramagnetic effects, which allowed to observe changes in contact and dipolar shifts. Also, variable concentration and temperature experiments provided new insights in the axial reactivity and exchange equilibria, respectively.
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