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SUMMARY:Andreas Meyer
DESCRIPTION:Using 19F to Study Paramagnetic Biomolecules at the Nanoscale with Electron Nuclear Double Resonance \n19F labels are well established in NMR spectroscopy and allow accessing specific molecular information within biomolecular frameworks such as proteins. In case of radical enzymes or paramagnetic metalloproteins\, the molecular structure around the paramagnetic center within a radius of up to ca. 20 Å can be investigated by electron nuclear double resonance (ENDOR) spectroscopy\, benefitting from the high electron spin polarization. The method can also be applied to diamagnetic molecules if paramagnetic spin labels are used.In this talk\, the basic principles of 19F ENDOR will be presented in an instructive fashion\, followed by an overview of how the method developed in the past five years. As an application\, the use of 19F ENDOR in studying the proton coupled electron transfer of ribonucleotide reductase\, a tetrameric radical enzyme with more than 2200 residues\, will be showcased. \n8:00 AM California or 11:00 AM Boston or 5:00 PM Paris or 8:30 PM Delhi
URL:https://www.globalnmr.org/upcoming-discussions/andreas-meyer/
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