High-dimensional spectroscopy and other tools to tackle complexity in biological solid-state NMR
Fast (> 60 kHz) magic-angle spinning provides high sensitivity and narrowed 1H linewidths, however, spectral analysis of large proteins are often severely hampered by peak overlap and/or ambiguity, depending on system size and sample quality. In this tutorial lecture, I will discuss several experimental techniques to address this, namely high-dimensional (4D, 5D) spectroscopy with non-uniform sampling, projection spectroscopy and time-shared acquisition. Additionally, I will explore selected tools for automation of resonance assignment and present early data on protein dynamics measurement as pseudo-4D series.
8:00 AM California or 11:00 AM Boston or 5:00 PM Paris or 9:30 PM Delhi