DNP-enhanced solid-state NMR spectroscopy of chromatin polymers

Nesreen Elathram – @NesreenElathram

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— Nesreen (@NesreenElathram) August 3, 2022

Chromatin is a complex biological polymer that packages DNA and regulates access to the genetic information in eukaryotic cells. Chromatin structural biology is hampered by the size, the heterogeneity and the presence of both dynamic and rigid components in the polymer. Here, we use DNP to enhance the ssNMR sensitivity of chromatin and investigate the effect of cryogenic temperatures on chromatin structure and spectral resolution.