Structural Analysis of Human Prion Protein using NMR Methodologies Under Cell Mimicking Conditions

Manoj Madheswaran – @Mano_Sampath

Structural analysis of Human Prion protein using NMR methodologies under cell mimicking conditions (Ficoll 70 Crowding agent). We did a comparative study of Prion under standard buffer condition and crowding condition to study in-depth detail about prion folding#GlobalnmrTC2022 pic.twitter.com/xMLd34iBMP

— Manoj M Sampath (@Mano_Sampath) August 3, 2022

Using Nuclear Magnetic Resonance (NMR) spectroscopy to investigate conformational equilibria of human prion protein in cell mimicking conditions, using ficoll, an inert crowding agent. Using CD and NMR methodologies, we carried out an atom by atom analysis of human prion thermal unfolding in the presence of ficoll.