Structural characterization by NMR spectroscopy of a DnaX mini-intein derived from Spirulina platensis

Soumendu Boral – @BoralSoumendu

Hello NMR community! Delighted to share our work on structural and dynamic characterization of a novel intein enzyme using solution NMR spectroscopy at @global_nmr. #GlobalnmrTC2022 #NMRchat #biologicalNMR #proteinsplicing @nmrfam @buildmodels@SomDe2015 @TheWoonghee @IITKgp pic.twitter.com/htI3Lox3Xj

— Soumendu Boral (@BoralSoumendu) August 3, 2022

Inteins perform protein splicing reaction. We solved solution NMR structure (PDB code: 7CFV) of a 136-residue DnaX mini-intein. The NMR structural ensemble has backbone and heavy atom RMSD of 0.27 and 0.52 Å, respectively. NMR-based HX and 15N-relaxation experiments showed the presence of a very stable core and conserved motions.