Biological NMR

Deepraj Negi – @ThevoiceDeepraj

Thrilled to present a poster titled "Mapping Molecular Interactions of G-Quadruplex Structure" at the #GlobalnmrTC2022. Thanks to @bwajtweeting for designing the project and @RR_Sannapureddi for scientific inputs. @chm_iiserb @iiserbhopal @hemakshizzaa #NMR pic.twitter.com/5yn9XNFW5k

— Deepraj_Negi (@ThevoiceDeepraj) August 3, 2022

G-quadruplexes (G4s) are known to be present in critical regions of the genome and their presence thus implicates them in several crucial biological processes. G4 is modulated by multiple factors, importantly by the primary nucleotides and the nature and concentration of the cation present. By altering the ions, loop length, and changing guanosine to inosine, the goal of this project is to determine the molecular interactions of the G-quad.

Sophie Marianne Korn – @Sophie__Korn

Delighted to present my work at #GlobalnmrTC2022! In the Schlundt group @lab_nmr in Frankfurt @goetheuni, we use #NMR to study RNA-protein complexes. Take a glimpse at our work on #SARSCoV2 Nucleocapsid NTD and its RNA preferences, following a short intro on our standard tools. pic.twitter.com/zYgtaTboTX

— Sophie Korn (@Sophie__Korn) August 3, 2022

How the SARS-CoV-2 nucleocapsid protein (N) recognizes specific RNA motifs remains unanswered. We use NMR to analyze the interactions of N’s N-terminal RNA-binding domain with RNA elements in the viral genomic RNA. The domain uses defined sensory residues to read intrinsic signatures of preferred RNA elements from available motifs.

Sofia Mariasina – @SMariasina

I'm happy to present our work "Vitamin Status Assessment by NMR" in the 2022 Global NMR Twitter Conference!

My colleagues: Laricheva D, Aysin K, Varaeva Yu, Starodubova A, Ivlev V and @v_polshakov#GlobalnmrTC2022 #MoscowStateUniversity pic.twitter.com/5TnesBguxP

— Sofia Mariasina (@SMariasina) August 3, 2022

We try to assess vitamin status by 1H NMR using blood and urine samples of healthy volunteers who takes vitamins D, C, B1, B6, B9, or B12 as a supplementation to their diet. Some biomarkers of a person’s vitamin status were found, which can be used for personalized vitamin therapy.

Rajesh Kumar Reddy Sannapureddi – @RR_Sannapureddi

How does duplex impact G4. Look at my poster for exciting work on Quadruplex-duplex.#GlobalnmrTC2022

Thanks @bwajtweeting for guidance and @iiserbhopal @serbonline for facilities and funding. pic.twitter.com/VSwP7ct5Iv

— Rajesh Sannapureddi (@RR_Sannapureddi) August 3, 2022

So far G-quadruplexes (G4) were studied individually, structural characteristics of G4s in presence of duplex is not well characterized. G4’s with duplex on 5’/3′ are designed to mimic genome. Here, we present atomistic characterization G4 and impact of 5’/3’ duplex and linker length using NMR and molecular dynamic (MD) simulations.

Manoj Madheswaran – @Mano_Sampath

Structural analysis of Human Prion protein using NMR methodologies under cell mimicking conditions (Ficoll 70 Crowding agent). We did a comparative study of Prion under standard buffer condition and crowding condition to study in-depth detail about prion folding#GlobalnmrTC2022 pic.twitter.com/xMLd34iBMP

— Manoj M Sampath (@Mano_Sampath) August 3, 2022

Using Nuclear Magnetic Resonance (NMR) spectroscopy to investigate conformational equilibria of human prion protein in cell mimicking conditions, using ficoll, an inert crowding agent. Using CD and NMR methodologies, we carried out an atom by atom analysis of human prion thermal unfolding in the presence of ficoll.

Kenneth Marincin – @KennyMarincin

Check out our group's latest research on the importance of protein dynamics in NRPS Cy domains. Happy to share this poster even in a virtual setting. A fantastic application of NMR methodologies. #GlobalnmrTC2022 pic.twitter.com/cFP2BUZOaF

— Kenny Marincin (@KennyMarincin) August 3, 2022

Nonribosomal peptide synthetase (NRPS) cyclization (Cy) domains are central to the biosynthesis of many medicinal and virulent compounds. Using solution NMR and mutagenesis, we showed that global dynamics convey an allosteric response to a partner domain which may be key to communication and substrate recognition in Cy domains.

Jobst Liebau – @Jobst_Liebau

Solution-state NMR of large protein complexes is difficult yet of huge interest. We demonstrate how it can be done on an asymmetric 410 kDa decamer, using methyl- and 19F-labeling. We validate structures and measure dynamics and transient RNA substrate interactions, thus expanding solution-state NMR into the realm of cryo-EM structures.

Link

Joao Medeiros Silva – @JMSism

The Envelope Protein of SARS-CoV-2 is a viroporin that may induce severe inflammation for COVID. ssNMR+19F gave us the structure and an aromatic gating mechanism regulating this viroporin (💊🎯), induced by calcium and acidic pH. @MeiHongLab #GlobalnmrTC2022 +poster below pic.twitter.com/sXF0MBgces

— Joao Medeiros Silva (@JMSism) August 4, 2022

The Envelope Protein of SARS-CoV-2 forms a viroporin that induces inflammation and acute respiratory symptoms associated with COVID-19. Using ssNMR, we describe the structure and gating mechanisms governing this viroporin. 19F-NMR experiments revealed an aromatic network regulating the channel, which adopts an open-configuration at acidic pH and presence of calcium.