Tobias Schubeis – @TSchubeis
The CorA channel is the major Mg2+-influx pathway in prokaryotes. The mechanistic details of the channel gating and the transport of the metal ions are still not entirely understood. Here we investigate the dynamics of CorA in DMPC lipid bilayers with 1H-detected solid-state NMR at 100 kHz MAS.
Mummidivarapu Varkrishna – @VarkrishnaM
The concept of photocatalytic water splitting is revolutionary however the applications aren’t so feasible to scale up. This research area has largely been dominated by the inorganic materials.However, organic ones are included every now and then. But the extent is less. The exploration and preparation of composites more based on the organic substances whereas the inorganic ones can be always supporting is a huge ocean of possibilities. And NMR is quite a powerful technique to explore it!
Deepraj Negi – @ThevoiceDeepraj
G-quadruplexes (G4s) are known to be present in critical regions of the genome and their presence thus implicates them in several crucial biological processes. G4 is modulated by multiple factors, importantly by the primary nucleotides and the nature and concentration of the cation present. By altering the ions, loop length, and changing guanosine to inosine, the goal of this project is to determine the molecular interactions of the G-quad.
Sophie Marianne Korn – @Sophie__Korn
How the SARS-CoV-2 nucleocapsid protein (N) recognizes specific RNA motifs remains unanswered. We use NMR to analyze the interactions of N’s N-terminal RNA-binding domain with RNA elements in the viral genomic RNA. The domain uses defined sensory residues to read intrinsic signatures of preferred RNA elements from available motifs.
Sofia Mariasina – @SMariasina
We try to assess vitamin status by 1H NMR using blood and urine samples of healthy volunteers who takes vitamins D, C, B1, B6, B9, or B12 as a supplementation to their diet. Some biomarkers of a person’s vitamin status were found, which can be used for personalized vitamin therapy.
Rajesh Kumar Reddy Sannapureddi – @RR_Sannapureddi
So far G-quadruplexes (G4) were studied individually, structural characteristics of G4s in presence of duplex is not well characterized. G4’s with duplex on 5’/3′ are designed to mimic genome. Here, we present atomistic characterization G4 and impact of 5’/3’ duplex and linker length using NMR and molecular dynamic (MD) simulations.
Nesreen Elathram – @NesreenElathram
Chromatin is a complex biological polymer that packages DNA and regulates access to the genetic information in eukaryotic cells. Chromatin structural biology is hampered by the size, the heterogeneity and the presence of both dynamic and rigid components in the polymer. Here, we use DNP to enhance the ssNMR sensitivity of chromatin and investigate the effect of cryogenic temperatures on chromatin structure and spectral resolution.
Manoj Madheswaran – @Mano_Sampath
Using Nuclear Magnetic Resonance (NMR) spectroscopy to investigate conformational equilibria of human prion protein in cell mimicking conditions, using ficoll, an inert crowding agent. Using CD and NMR methodologies, we carried out an atom by atom analysis of human prion thermal unfolding in the presence of ficoll.
Kenneth Marincin – @KennyMarincin
Nonribosomal peptide synthetase (NRPS) cyclization (Cy) domains are central to the biosynthesis of many medicinal and virulent compounds. Using solution NMR and mutagenesis, we showed that global dynamics convey an allosteric response to a partner domain which may be key to communication and substrate recognition in Cy domains.
Jobst Liebau – @Jobst_Liebau
Solution-state NMR of large protein complexes is difficult yet of huge interest. We demonstrate how it can be done on an asymmetric 410 kDa decamer, using methyl- and 19F-labeling. We validate structures and measure dynamics and transient RNA substrate interactions, thus expanding solution-state NMR into the realm of cryo-EM structures.
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